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Image Search Results
Journal: Journal of Clinical Medicine
Article Title: Early Cellular and Humoral Responses Developed in Oncohematological Patients after Vaccination with One Dose against COVID-19
doi: 10.3390/jcm11102803
Figure Lengend Snippet: DCC and viral neutralization activity against SARS-CoV-2 infected cells of PBMCs of individuals with OHD before and after receiving one-dose vaccine. ( A ) The capacity of PBMCs from individuals with OHD to eliminate SARS-CoV-2-infected Vero E6 cells was determined by quantifying the production of Renilla (RLUs) after co-culture for 1 h. ( B ) DCC was assessed by measuring the activity of caspasa-3 in SARS-CoV-2-infected Vero E6 cells co-cultured with PBMCs from individuals with OHD, in comparison with healthy donors. Each dot in the graphs corresponds to mean ± SEM. Statistical significance between groups was calculated using one-way ANOVA test.
Article Snippet:
Techniques: Neutralization, Activity Assay, Infection, Co-Culture Assay, Cell Culture, Comparison
Journal: Journal of medical virology
Article Title: Neutralizing antibody response after immunization with a COVID-19 bivalent vaccine: Insights to the future.
doi: 10.1002/jmv.29416
Figure Lengend Snippet: FIGURE 2 Neutralization titer according to SARS‐CoV‐2 strain. Neutralization assays using the wild‐type SARS‐CoV‐2, the original Omicron variant (BA.1), and its subvariants FE.1.2 or BQ.1.1 were carried out. In brief, cell monolayers (5 × 104 Vero CCL‐81 cells/well) in 96‐well culture plates were exposed to 1 × 103 TCID50/mL of SARS‐CoV‐2 Wuhan strain—wild type or Omicron subvariants (BA.1, FE.1.2, BQ.1.1) that were previously incubated with 1:20–1:1280 twofold diluted, heat‐inactivated human serum samples, in a final volume of 150 µL. After 72 h of incubation, the plates were evaluated microscopically for the presence of characteristic SARS‐CoV‐2 CPEs. The absence of CPEs in the 1:20 diluted sample was considered a positive result for the presence of neutralizing antibodies against SARS‐CoV‐2. Comparisons between neutralization titers measured in samples from individuals immunized only with monovalent vaccines and those who received a booster shot with the bivalent vaccine are shown with regard to the wild‐type SARS‐CoV‐2 (A), Omicron BA.1 (B) and its subvariants FE.1.2 (C), and BQ.1.1 (D). In addition, neutralization titers with regard to each SARS‐CoV‐2 strain were compared considering values of samples from volunteers immunized only with monovalent vaccines (E) or those who received a booster shot with the bivalent vaccine (F). Statistical significance was set as p ≤0.05. *p ≤0.05; **p ≤0.01; ***p ≤0.001; ****p ≤0.0001. CPEs, cytopathic effects; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2; TCID, tissue culture infectious dose.
Article Snippet:
Techniques: Neutralization, Variant Assay, Incubation, Vaccines